Mon-Fri 9:30 - 18:00

+7 985 333-51-99

Moscow, Godovikova str. 9-1
Russian version (RU)

Choose an enzyme or reaction type


Diamant Taq A polymerase


Hot start Taq DNA polymerase with temperature of activation at ≥ 37 °C. Stable at room temperature.

Diamant Taq D polymerase


Hot start Taq DNA polymerase with temperature of activation at ≥ 60 °C.

Diamant Taq polymerase


Recombinant Taq DNA polymerase

Proteinase K


Enzyme for proteins hydrolysis during nucleic acids isolation and purification.

Uracil-DNA glycosylase

New product

Enzyme for prevention of false positive results in PCR.

RevertM revertase


Thermostable M-MuLV reverse transcriptase.

Blitz DNA polymerase


Thermostable hybrid polymerase for high-precision PCR.

5X One-Step RT-PCR Mastermix


Ready-to-use mixture for one step RT-PCR.

5X Fast Probe qPCR Mastermix


Ready-to-use mixture for qPCR. Stable at +4 °C.

5X Fast Probe qPCR Mastermix U

New product

Ready-to-use mixture for qPCR with resistance to false positive results. Stable at +4 °C.

  • Reverse transcription
  • Long and accurate PCR (LA PCR)
  • Hot start
  • qPCR with Taqman probes
  • Fast PCR
  • PCR in the presence of inhibitors
  • Multiplex PCR

To order enzymes just call us!

+7 985 333-51-99

or send an e-mail

order@belbiolab.ru

About company


BelBioLab LLC develops and produces enzymes for molecular biology. Our portfolio includes different DNA- and RNA-dependent polymerases, ready-for-use mixes for qPCR and RT-qPCR, etc. We give particular attention to standardization and quality control of products. We can optimize the methods of quality control, the concentration of the enzyme, the composition of the buffer systems and other characteristics of the products to the needs of the customer.

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Diamant TaqA polymerase


Diamant TaqA DNA polymerase is a mixture of high-purified recombinant Taq DNA polymerase and a specific aptamer.

Diamant TaqA DNA polymerase does not have enzymatic activity under PCR conditions, which leads to an increase in the specificity and sensitivity of the reaction. TaqA DNA polymerase is stay inactive during the incubating with the substrate for 1 hour at 37 °C. Activation of the enzyme occurs automatically during the first cycle of DNA denaturation.

Diamant TaqA DNA polymerase catalyzes DNA synthesis in the direction from the 5'- to 3'-end of the growing chain. Enzyme does not have detectable corrective 3'-5'-exonuclease activity but it has 5'-3'-exonuclease activity. In addition, Diamant TaqA DNA polymerase has deoxynucleotide transferase activity, which results in the addition of an additional residue of dA to the 3 'end of the PCR product.

Diamant TaqA DNA polymerase provides a high yield of the PCR product using different DNA matrices, and the probability of occurrence of non-specific amplification is minimized. The enzyme allows the amplification of DNA fragments up to 5,000 bp.

Diamant TaqA polymerase in original buffer can be stored at room temperature (25 °C) for 1 month without significant changes in activity and inactivation level.

APPLICATION AREA

Shipping and Storage

Storage buffer:

20 mM TRIS-HCl (pH 8,0), 100 mM KCl, 0,1 mM EDTA, 1 mM DTT, 50 % glycerol, 1 % Tween-20.

Storage of Diamant TaqA DNA polymerase is allowed at 25 °C for 30 days and at -20 °C for 2 years.

Transportation

Transportation of Diamant TaqA DNA polymerase is carried out at ambient temperature, but not above 25 °C (up to 30 days) or at +4 °C for a long time.

Documents

1 150 EUR

Quantity: 50 000 units

In Stock

8 300 EUR

Quantity: 500 000 units

In Stock

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Diamant TaqD polymerase


Diamant TaqD DNA is a mixture of highly purified recombinant Taq DNA polymerase and specific monoclonal antibodies to this enzyme. Due to the presence of the monoclonal antibodies, the DNA polymerase does not have enzymatic activity under the conditions of preparation of the PCR mixture, which leads to an increase in the specificity and sensitivity of the reaction.

Diamant TaqD DNA polymerase is stay inactive during the incubating with the substrate for 1 hour at 55 °C. Activation of the enzyme occurs automatically during the first cycle of DNA denaturation.

Diamant TaqD DNA polymerase catalyzes DNA synthesis in the direction from the 5'- to 3'-end of the growing chain. Enzyme does not have detectable corrective 3'-5'-exonuclease activity but it has 5'-3'-exonuclease activity. In addition, Diamant TaqD DNA polymerase has deoxynucleotide transferase activity, which results in the addition of an additional residue of dA to the 3 'end of the PCR product.

Diamant TaqD DNA polymerase provides a high yield of the PCR product using different DNA matrices, and the probability of occurrence of nonspecific amplification is minimized. The enzyme allows the amplification of DNA fragments up to 5,000 bp.

Application

Shipping and Storage

Storage buffer:

20 mM TRIS-HCl (pH 8,0), 100 mM KCl, 0,1 mM EDTA, 1 mM DTT, 50 % glycerol, 1 % Tween-20.

Storage of Diamant TaqD DNA polymerase is allowed at -20 °C for 1 years.

Transportation

Transportation of Diamant TaqD DNA polymerase is carried out at ambient temperature (below 25°C) up to 7 days, at +4 °С up to 30 days, at a temperature of less than -16 °C for a long time

Documents

1 650 EUR

Quantity: 50 000 units

In Stock

11 500 EUR

Quantity: 500 000 units

In Stock

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Diamant Taq polymerase


Diamant Taq-DNA polymerase is a 94 KDa thermostable protein isolated from a recombinant E. coli strain bearing the polA gene of the Thermus aquaticus YT1 polymerase. The enzyme is highly purified by affinity and anion-exchange chromatography and can be supplied in concentrated form up to 500 units/μl. Diamant Taq DNA polymerase can be supplied in the buffer without glycerin and detergents for the subsequent lyophilization.

Application:

Routine PCR.

1 150 EUR

Quantity: 100 000 units

In Stock

8 300 EUR

Quantity: 1 000 000 units

In Stock

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Proteinase K


Proteinase K is a 28.9 kDa serine protease produced by the recombinant Pichia pastoris strain carrying the Proteinase K gene of Tritirachium album. The enzyme has a broad substrate specificity for a large number of proteins. Proteinase K hydrolysis polypeptide chain predominantly at sites containing nonpolar amino acids.

APPLICATION AREA:

Nucleic acids isolation.

1 150 EUR

Quantity: 5 gram

In Stock

2 150 EUR

Quantity: 10 gram

In Stock

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Uracil-DNA glycosylase


Uracil-DNA glycosylase (UDG) catalyzes the release of free uracil from uracil-containing DNA. UDG effectively hydrolyzes uracil from single- and double-stranded DNA. UDG is used in PCR to prevent the appearance of false positive results, which are caused by contamination of the reaction PCR mixture with products of previously performed PCR.

APPLICATION AREA:

Prevention of false positive results in PCR.

1 200 EUR

Quantity: 50 000 units

In Stock

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RevertM revertase


RevertM revertase is a genetically modified reverse transcriptase of the Moloney Mouse Leukemia Virus (MMLV), which performs the synthesis of a complementary strand of DNA (cDNA) on a single-stranded RNA template. Due to the presence of several mutations, the enzyme has inhibited activity of RNase H and increased thermostability (the optimal temperature for the enzyme is 50 °C, the enzyme remains active at a temperature of up to 65 °C).

RevertM revertase compared to wild-type MMLV provides higher cDNA yield, allows longer fragment amplification (up to 12,000 bp), has improved efficacy with GC-rich RNA matrices, and higher productivity.

APPLICATION AREA

690 EUR

Quantity: 100 000 units

In Stock

4 850 EUR

Quantity: 1 000 000 units

In Stock

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Blitz DNA polymerase


Blitz DNA polymerase is a mutated variant of Pfu DNA polymerase with an increased processivity, accuracy and increased resistance of the enzyme to the high ionic strength of the solution. In addition, Blitz DNA polymerase is resistant to inhibitors and can be used in "direct" PCR without preliminary purification of the matrix (resistant to 10% of the blood). The enzyme is not inhibited by dUTP.

Blitz DNA polymerase catalyzes DNA synthesis in the direction from the 5'- to 3'-end of the growing chain. Enzyme does not have detectable corrective 3'-5'-exonuclease activity but it has 5'-3'-exonuclease activity.

Blitz DNA polymerase has an increased rate of DNA synthesis (up to 4,000 bp per minute) and is capable of amplifying fragments longer than 10,000 bp.

Blitz DNA polymerase is supplied with a 2.5X buffer, which is optimized for DNA amplification under standard conditions, as well as a solution of 50 mM MgCl2 for optimization of the amplification conditions. The supplied buffer contains dNTPs at a concentration of 0.2 mM (1-fold) and MgCl2 at a concentration of 2 mM. In most cases, these quantities are optimal.

APPLICATION AREA

Shipping and Storage

Blitz DNA polymerase is shipped at ambient temperature (below 25°C) up to 7 days, at +4 °С up to 30 days, at a temperature of less than -16°C for a long time.

Blitz DNA polymerase should be stored at -20°C, and has a shelflife of 2 years when stored properly under these conditions.

Documents

1 650 EUR

Quantity: 10 000 units

In Stock

8 750 EUR

Quantity: 50 000 units

In Stock

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5X One-Step RT-PCR Mastermix


5X One-Step RT-PCR Mastermixis a universal ready-to-use reagent for the synthesis of cDNA followed by quantitative and/or qualitative PCR with real-time detection of the results (hereinafter qPCR). The synthesis of cDNA and qPCR is carried out in the single tube. 5X One-Step RT-PCR Mastermix contains all basic components, including a revertase with a "warm start", a thermostable DNA polymerase with a "hot start", dNTP and an optimized buffer with enhancer and stabilizer.

5Х One-Step RT-PCR Mastermix provides reproducible results with high sensitivity (single copies of RNA).

5X One-Step RT-PCR Mastermix contains genetically modified MMLV revertase with suppressed RNase H activity and increased thermal stability, as well as improved efficacy with GC-rich RNA matrices, which leads to an increase in cDNA yield. In addition, 5X One-Step RT-PCR Mastermix contains an antibody-inactivated DNA polymerase, which does not have enzymatic activity under the conditions of preparation of the reaction mixture and reverting, which leads to an increase in the specificity and sensitivity of the reaction. Enzyme activation occurs automatically during the first DNA denaturation cycle at a temperature above 55 °C. The premix provides multiplex PCR (up to 4 simultaneously-detected targets). When setting up multiplex reactions, optimization of the number of oligonucleotides and a probe for each target, as well as the time of reverting, is recommended.

5Х One-Step RT-PCR Mastermix can be stored at temperatures up to + 4 °C for 90 days without loss of activity and specificity, and it can survive for at least 10 cycles of freeze/thaw.

APPLICATION AREA

Shipping and Storage

5Х One-Step RT-qPCR Mastermix is shipped at ambient temperature (below 25°C) up to 5 days, at +4°С up to 30 days, at a temperature of less than -16 °C for a long time.

5Х One-Step RT-PCR Mastermix should be stored at -20° and has a shelf life of 12 months when stored properly under these conditions. The product is stable for at least 10 freeze/thaw cycles, and for short-term storage may be stored at 4°C, for up to 1 month.

To maintain optimal performance of the 5Х One-Step RT-qPCR Mastermix we recommend storing master mix as appropriately sized aliquots in sterile, polypropylene tubes

5Х One-Step RT-PCR Mastermix хранится при –20 оС.

Documents

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5X Fast Probe qPCR Mastermix


5X Fast Probe qPCR Mastermix is a universal ready-to-use reagent for quantitative and/or qualitative PCR with real-time detection of the results (hereinafter qPCR).

5x Fast Probe qPCR Mastermix provides reproducible results with high sensitivity (single copies of DNA) when determining the amount of genomic, plasmid, viral and cDNA.

5x Fast Probe qPCR Mastermix contains an inactivated DNA polymerase that does not have enzymatic activity under PCR preparation conditions, which leads to increased specificity and sensitivity of the reaction. Enzyme activation occurs automatically during the first DNA denaturation cycle at a temperature above 40 °C.

5x Fast Probe qPCR Mastermix provides multiplex qPCR (up to 4 simultaneously-detected targets). When setting up multiplex reactions, optimization of the number of oligonucleotides and a probe for each target is recommended.

5x Fast Probe qPCR Mastermix can be stored at temperatures up to + 4 °C for 180 days without loss of activity and specificity, and it can survive for at least 10 cycles of freeze/thaw.

APPLICATION AREA

qPCR with double-labeled probes;

PCR multiplex.

Shipping and Storage

5x Fast Probe qPCR Mastermix is shipped at ambient temperature (below 25°C) up to 5 days, at +4°С up to 180 days, at a temperature of less than -16 °C for a long time.

5x Fast Probe qPCR Mastermix should be stored at -20° and has a shelf life of 2 years when stored properly under these conditions. The product is stable for at least 10 freeze/thaw cycles, and for short-term storage may be stored at 4°C, for up to 1 month.

To maintain optimal performance of the 5x Fast Probe qPCR Mastermix we recommend storing master mix as appropriately sized aliquots in sterile, polypropylene tubes .

1 100 EUR

Quantity: 1 000 reactions

In Stock

4 450 EUR

Quantity: 50 000 reactions

In Stock

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Урацил-ДНК-гликозилаза


Урацил-ДНК-гликозилаза (UDG) катализирует высвобождение свободного урацила из урацил-содержащих ДНК. UDG эффективно гидролизует урацил из одно- и двуцепочечных ДНК. Используется в ПЦР смесях для предотвращения появления ложноположительных результатов, которые в свою очередь вызваны контаминацией реакционной ПЦР-смеси продуктами ранее выполненной ПЦР.

Состав Урацил-ДНК-гликозилаза – 5 ед/мкл;
Источник Рекомбинантный штамма E.coli.
Единица активности За одну единицу активности принимали количество фермента, необходимое для высвобождения 60 пмолей урацила за 1 мин из двуцепочечной урацил-содержащей ДНК при 37°С.
Буфер для хранения и разведения 20 mM Tris-HCl (pH 8.8); 100 mM KCl; 0,5 mM EDTA; 0,5% Tween 20; 50% глицерин.
Область применения В ПЦР и ПЦР-РВ для предотвращения появления ложноположительных результатов.
Условия хранения UDG хранится при температурах от -20°С до 4°С.
Условия транспортировки UDG можно транспортировать при комнатной температуре.
Срок годности 1 год со дня проведения тестирования (хранение при -20°C).

по запросу

Объем поставки: по запросу

Срок поставки: от 5 рабочих дней.

Состав

Урацил-ДНК-гликозилаза – 5 ед/мкл;

Источник

Рекомбинантный штамма E.coli.

Единица активности

За одну единицу активности принимали количество фермента, необходимое для высвобождения 60 пмолей урацила за 1 мин из двуцепочечной урацил-содержащей ДНК при 37°С.

Буфер для хранения и разведения

20 mM Tris-HCl (pH 8.8); 100 mM KCl; 0,5 mM EDTA; 0,5% Tween 20; 50% глицерин.

Область применения

В ПЦР и ПЦР-РВ для предотвращения появления ложноположительных результатов;

Условия хранения

UDG хранится при температурах от -20°С до 4°С.

Условия транспортировки

UDG можно транспортировать при комнатной температуре.

Срок годности

1 год со дня проведения тестирования (хранение при -20°C).

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5Х One-Step RT-PCR Mastermix


5Х One-Step RT-PCR Mastermix представляет собой универсальный готовый к использованию реагент для получения кДНК с последующей количественной и/или качественной ПЦР с детекцией результатов в режиме реального времени (далее ОТ-ПЦР). При этом синтез кДНК и ПЦР осуществляется в одной пробирке. 5Х One-Step RT-PCR Mastermix содержит все основные реагенты, включая ревертазу c «теплым стартом», термостабильную ДНК-полимеразу с «горячим стартом», дНТФ и оптимизированный буфер.

Использование 5Х One-Step RT-PCR Mastermix для проведения ОТ-ПЦР обеспечивает получение воспроизводимых результатов с высокой чувствительностью (единичные копии РНК).

В состав 5Х One-Step RT-PCR Mastermix входит генетически модифицированная ревертаза MMLV с подавленной активностью РНКазы Н и повышенной термостабильностью, а также улучшенной эффективностью при использовании GC-богатых РНК-матриц, что приводит к увеличению выхода кДНК.

Кроме того, в состав 5Х One-Step RT-PCR Mastermix входит инактивированная антителами ДНК-полимераза, которая не обладает ферментативной активностью в условиях приготовления реакционной смеси и ревертирования, что приводит к повышению специфичности и чувствительности реакции. Активация фермента происходит автоматически в процессе первого цикла денатурации ДНК при температуре выше 55°C.

Премикс обеспечивает проведение мультиплексных ПЦР-РВ (до 4 одновременно-детектируемых мишеней). При постановке мультиплексных реакций рекомендуется оптимизация количества олигонуклеотидов и зонда для каждой мишени, а также времени ревертирования.

Область применения
  • Мультиплекс ОТ-ПЦР
  • Рутинная ОТ-ПЦР
  • Низкокопийная ОТ-ПЦР
  • Высокоспецифичная ОТ-ПЦР
  • ОТ-ПЦР с двумеченными зондами
Транспортировка Транспортирование 5Х One-Step RT-PCR Mastermix осуществляется при температуре окружающей среды, но не выше 25°С (до 2 суток), при температуре +4°С (до 10 суток) или при температуре менее –18°С продолжительное время.
Условия хранения 5Х One-Step RT-PCR Mastermix хранится при –20°С. Допускается хранение 5Х One-Step RT-PCR Mastermix при температуре +2 – +8 °С в течение 30 суток.
Особенности хранения Допускается заморозка/оттаивание 5Х One-Step RT-PCR Mastermix не более 10 раз. Рекомендуется приготовить аликвоты 5Х One-Step RT-PCR Mastermix удобного объема для однократного использования.
Срок годности 1 год.
Документы

цена по запросу

Объем поставки: по запросу

Срок поставки: 5 рабочих дней.

Область применения

  • Мультиплекс ОТ-ПЦР
  • Рутинная ОТ-ПЦР
  • Низкокопийная ОТ-ПЦР
  • Высокоспецифичная ОТ-ПЦР
  • ОТ-ПЦР с двумеченными зондами

Документы

Транспортировка

Транспортирование 5Х One-Step RT-PCR Mastermix осуществляется при температуре окружающей среды, но не выше 25°С (до 2 суток), при температуре +4°С (до 10 суток) или при температуре менее –18°С продолжительное время.

Условия хренения

5Х One-Step RT-PCR Mastermix хранится при –20°С. Допускается хранение 5Х One-Step RT-PCR Mastermix при температуре +2 – +8 °С в течение 30 суток.

Особенности хранения

Допускается заморозка/оттаивание 5Х One-Step RT-PCR Mastermix не более 10 раз. Рекомендуется приготовить аликвоты 5Х One-Step RT-PCR Mastermix удобного объема для однократного использования.

Срок годности

1 год.

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5X Fast Probe qPCR Mastermix U


5x Fast Probe qPCR Mastermixis a universal ready-to-use reagent for quantitative and/or qualitive PCR with real-time detection of the results (hereinafter qPCR).

The use of an inactivated DNA polymerase, which does not have enzymatic activity under the conditions of preparation of the PCR mixture, leads to an increase in the specificity and sensitivity of the reaction. Enzyme activation occurs automatically during the first DNA denaturation cycle. Presence of dUTP in the reaction mixture leads to the fact that all the amplification products will contain uracil in their composition. In this case, UDG in the new PCR run effectively hydrolyses uracil from single- and double-stranded DNA molecules during the reaction setup, thus preventing carry-over contamination by amplicon of preceding PCR and obtaining false positive results. UDG is inactivated during the first DNA denaturation cycle and does not interfere with the amplification of the products of the current reaction.

The use of 5X Fast Probe qPCR Mastermix U for qPCR provides reproducible results with high sensitivity (single copies of DNA) when determining the amount of genomic, plasmid, viral and cDNA.

5x Fast Probe qPCR Mastermix provides multiplex qPCR (up to 4 simultaneously detected targets). When setting up multiplex reactions, optimization of the number of oligonucleotides and a probe for each target is recommended.

5x Fast Probe qPCR Mastermix can be stored at temperatures up to + 4 ° C for 180 days without loss of activity and specificity, and it can survive for at least 10 cycles of freeze/thaw.

APPLICATION AREA

qPCR with double-labeled probes;

PCR multiplex;

prevention of false positive results in PCR.

Shipping and Storage

5x Fast Probe qPCR Mastermix U is shipped at ambient temperature (below 25°C) up to 5 days, at +4°С up to 180 days, at a temperature of less than -16 °C for a long time.

5x Fast Probe qPCR Mastermix U should be stored at -20° and has a shelf life of 2 years when stored properly under these conditions. The product is stable for at least 10 freeze/thaw cycles, and for short-term storage may be stored at 4°C, for up to 1 month.

To maintain optimal performance of the 5x Fast Probe qPCR Mastermix U we recommend storing master mix as appropriately sized aliquots in sterile, polypropylene tubes

1 250 EUR

Quantity: 10 000 reactions

In Stock

4 950 EUR

Quantity: 50 000 reactions

In Stock

Закрыть окно [x]

5Х One-Step RT-PCR Mastermix


5X Fast Probe qPCR Mastermix U представляет собой универсальный готовый к использованию реагент для количественной и/или качественной ПЦР с детекцией результатов в режиме реального времени (далее ПЦР-РВ). 5x Fast Probe qPCR Mastermix содержит все основные компоненты ПЦР, включая термостабильную ДНК-полимеразу с «горячим стартом», дНТФ и оптимизированный буфер.

Использование 5x Fast Probe qPCR Mastermix при проведении ПЦР-РВ обеспечивает получение воспроизводимых результатов с высокой чувствительностью (единичные копии ДНК) при определении количества геномной, плазмидной, вирусной и кДНК.

В состав 5x Fast Probe qPCR Mastermix входит инактивированная ДНК-полимераза, которая не обладает ферментативной активностью в условиях приготовления ПЦР-смеси, что приводит к повышению специфичности и чувствительности реакции. Активация фермента происходит автоматически в процессе первого цикла денатурации ДНК при температуре выше 40°С.

5x Fast Probe qPCR Mastermix обеспечивает проведение мультиплексных ПЦР-РВ (до 4 одновременно-детектируемых мишеней). При постановке мультиплексных реакций рекомендуется оптимизация количества олигонуклеотидов и зонда для каждой мишени.

Область применения ПЦР-РВ с двумеченными зондами
Транпортировка Транспортирование 5x Fast Probe qPCR Mastermix осуществляется при температуре окружающей среды, но не выше 30°С (до 5 суток) или при температуре +4°С (до 30 суток).
Условия хранения Допускается хранение 5x Fast Probe qPCR Mastermix при температуре +2 – +8°С в течение 90 дней.
Особенности хранения Допускается заморозка/оттаивание 5Х One-Step RT-PCR Mastermix не более 10 раз.
Срок годности 1 год.
Документы

цена по запросу

Объем поставки: по запросу

Срок поставки: 5 рабочих дней.

Область применения

ПЦР-РВ с двумеченными зондами

Документы

Транспортировка

Транспортирование 5Х One-Step RT-PCR Mastermix осуществляется при температуре окружающей среды, но не выше 25°С (до 2 суток), при температуре +4°С (до 10 суток) или при температуре менее –18°С продолжительное время.

Условия хренения

5Х One-Step RT-PCR Mastermix хранится при –20°С. Допускается хранение 5Х One-Step RT-PCR Mastermix при температуре +2 – +8 °С в течение 30 суток.

Особенности хранения

Допускается заморозка/оттаивание 5Х One-Step RT-PCR Mastermix не более 10 раз. Рекомендуется приготовить аликвоты 5Х One-Step RT-PCR Mastermix удобного объема для однократного использования.

Срок годности

1 год.